Plasmid_Backbone
Gal4::EX-R
Part:BBa_K3271020:Design
Designed by: Setti Belhouari Group: iGEM19_uOttawa (2019-10-16)
Gal4::EX-RFP-SP pSB1K3 Type IIS Assembly
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2290
Illegal suffix found at 2346 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2290
Illegal SpeI site found at 2347
Illegal PstI site found at 2361
Illegal NotI site found at 2296
Illegal NotI site found at 2354 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2290
Illegal XhoI site found at 1026
Illegal XhoI site found at 2052
Illegal XhoI site found at 2337 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2290
Illegal suffix found at 2347 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2290
Plasmid lacks a suffix.
Illegal XbaI site found at 2305
Illegal SpeI site found at 2347
Illegal PstI site found at 2361 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2312
Illegal BsaI.rc site found at 2340
Design Notes
Please note the upstream and downstream Gal4 locus homologies are found outside the prefix and suffix, not within the multiple cloning site. This contribution consists of an updated pSB1K3 BioBrick backbone that makes the BioBrick plasmids compatible with cloning in both E. coli and S. cerevisiae. This plasmid backbone is compatible with traditional and Type IIS cloning.
Source
The Gal4 upstream and downstream homologies are comprised of 100 bp each from the promoter region and the terminator region of the Gal4 locus of S. cerevisiae, respectively. The RFP cassette is identical in sequence to BBa_J04450.